Hi Cedric,
I used these, but just for testing crystals. I was afraid that the
crystal might move in the loop. For testing it worked pretty good.
http://www.jenabioscience.com/cms/en/1/catalog/733_microrttrade_room_temperature_mounting_system.html
There might be more suppliers. Please send a summary to ccp4bb.
Best
Guenter
cedric bauvois wrote:
Dear CCP4ers,
in their paper entitled " Using cryoloops for X-ray data collection
from protein crystals at room temperature: A simple applicable method"
( *Journal of Crystal Growth*
<http://www.sciencedirect.com/science/journal/00220248>
Volume 281, Issues 2-4
<http://www.sciencedirect.com/science?_ob=PublicationURL&_tockey=%23TOC%235302%232005%23997189997%23601824%23FLA%23&_cdi=5302&_pubType=J&view=c&_auth=y&_acct=C000026678&_version=1&_urlVersion=0&_userid=532047&md5=9a4e7b2fc158c6d2396925c79d995e3d>,
1 August 2005, Pages 592-595.), the authors present a way to mount
crystals using "a cryoloop accompanied by a glass capillary cap" (see
abstract below).
Do you know if any commercial version of such system are now available ?
Abstract: Although cryoloops are now routinely used for X-ray data
collection from protein crystals in cryocooling condition, it is still
necessary to collect X-ray diffraction data from protein crystals at
room temperature under such circumstances as to find resolution limit
and/or to avoid damage of protein crystals at cryogenic temperature
(e.g. 100 K). Here, we show that a cryoloop, which is accompanied by a
glass capillary cap to maintain humid environment of crystal in the
cryoloop, can be used not only to examine protein or non-protein
crystals but also to collect X-ray diffraction data for structural
analysis from protein crystals at room temperature. The size of
cryoloop should be carefully chosen so that the crystal does not move
in the cryoloop. This crystal mounting method can be time-saving
compared to the traditional method to mount a crystal in a glass
capillary tube.
Many thanks
--
Dr. Cedric Bauvois
Cristallographie des protéines
Institut de Recherches Microbiologiques JM Wiame -IRMW
Av E. Gryzon 1, 1070 Brussels (Belgium)
tél: +32 (0)2 5273634
fax: +32 (0)2 5267273
--
***********************************
Priv.Doz.Dr. Guenter Fritz
Fachbereich Biologie
Sektion Naturwissenschaften
Universitaet Konstanz
http://www.biologie.uni-konstanz.de/fritz
Universitaetsstrasse 10
Postfach M665
D-78457 Konstanz
e-mail: [email protected]
Tel. Office: +49-(0)7531 88 3205
Tel. Lab : +49-(0)7531 88 3687
Fax: +49-(0)7531 88 2966
