Dear Amit, Follow this link to a nice little paper describing exactly this: http://journals.iucr.org/d/issues/2007/04/00/bw5188/bw5188.pdf In the hands of D'arcy and colleagues, seed stocks could be frozen at 193 K and withstand multiple freeze/thaw cycles without decreasing their nucleation effect. HTH, David
________________________________ From: CCP4 bulletin board on behalf of amit sharma Sent: Fri 1/23/2009 12:14 PM To: [email protected] Subject: [ccp4bb] storing crystal seeds Dear All, I intend to perform some seeding experiments with my protein. I have prepared the seeds using the seed bead kit from Hampton and was wondering if I could store the seed stock for future experiments. Is that recommended? If yes, then what temperature should I store these at? Also, can the seed stock take multiple(2-3) freeze-thaw cycles? I would be grateful if anybody could shed light on this. Many thanks, in advance -- Amit Sharma
