Dear All, I have a 9-kDa protein that crystallizes well. Since there is no structural homologue for this molecule, I intend to make Se-Met derivative of the protein. The molecule has no Met/Cys residues in its sequence. I wanted to know where in the sequence should I mutate, so that the folding/crystallizability of the protein is not compromised.
Any suggestions would be of great help. Thanks in advance, -- Amit Sharma, Ph.D. Research Associate, Department of Biology, University of York, YO10 5DD UK
