Hi Ed,
A bit late on the subject ....
I have collected atomic resolution data (around 0.97A) on both bovine
and porcine phospholipase A2 crystals which at
the time of data collection were between 10-16 years old.
Crystallization setup was liquid-liquid diffusion in glass capillaries.
Differently from the other stories, here there's no degradation
involved...just rock-solid Xtals.
In the paper we stated: 'Crystals are stable in the crystallization
solution...' which I guess well reflects their behavior.
Best wishes,
Roberto
On 5 Feb 2009, at 19:11, Edward Snell wrote:
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Dear All,
I was recently trying to find references on how age may degrade a
crystal, i.e. grow them and use them or preserve them as fresh as
possible. I seem to remember seeing a couple of papers on this but my
memory is fading and I have been unable to locate them. Can anyone jog
my memory or tell me if I'm imagining things? I've found plenty on
the
protein prep etc. but nothing on the crystal.
Thanks,
Eddie.
Edward Snell Ph.D.
Assistant Prof. Department of Structural Biology, SUNY Buffalo,
Hauptman-Woodward Medical Research Institute
700 Ellicott Street, Buffalo, NY 14203-1102
Phone: (716) 898 8631 Fax: (716) 898 8660
Email: esn...@hwi.buffalo.edu Telepathy: 42.2 GHz
Heisenberg was probably here! Crystallization, how quaint!
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---
Roberto Steiner
Randall Division of Cell and Molecular Biophysics
New Hunt's House
King's College London
Guy's Campus
London, SE1 1UL
Phone +44 (0)20-7848-8216
Fax +44 (0)20-7848-6435
e-mail roberto.stei...@kcl.ac.uk
