Artem,
Artem Evdokimov wrote:
Please note that osmotic shock extraction typically employs EDTA which is
obviously bad for IMAC.
This is not entirely correct.
I have used extracts with 5 mM EDTA for IMAC in the past.
If your IMAC column volume is large enough, only the top 1-2 mm will be
depleted of Me2+ (easily seen with Cu2+).
Moreover, it is always possible to add some Me2+ to your extract prior
to IMAC.
All this was published long ago (Biochemistry. 31: 2690-2702, 1992).
Nadir
--
Pr. Nadir T. Mrabet
Cellular & Molecular Biochemistry
INSERM U-724
Nancy University, School of Medicine
9, Avenue de la Foret de Haye, BP 184
54505 Vandoeuvre-les-Nancy Cedex
France
Phone: +33 (0)3.83.68.32.73
Fax: +33 (0)3.83.68.32.79
E-mail: [email protected]
