Dear Jothi, Here is an alternative.
Selection of membrane protein targets for crystallization using PFO-PAGE electrophoresis.<http://www.ncbi.nlm.nih.gov/pubmed/18982523?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum> Cleverley RM, Saleem M, Kean J, Ford RC, Derrick JP, Prince SM. Mol Membr Biol. 2008 Dec;25(8):625-30. Regards Vijay On Fri, Feb 13, 2009 at 3:30 AM, KN <[email protected]> wrote: > Dear all, > > I am wondering if anyone is working on Blue Natve PAGE or other alternate > methods. I need some help to troubleshoot my Native PAGE experiments. It > will be great if anyone can help me in this regard. I am working on > membrane > proteins which have pI around less than 7. i need to run Native PAGE for my > samples which are purified with nonionic detergents. When i tried to run > inhouse 4-16% gradient Native gel at running buffer pH of 8.9, the samples > are getting stuck at the wells itself. I just found out that invitrogen > Blue > Natvie PAGE may be a good option but due to the cost if anyone can suggest > me some other alternate methods, I will really appreciate it. > > Thanks in advance, > > regards, > > Jothi, >
