Dear guys, Sorry for the off-topic question.
After I have solved my strucutre, I have found my target ligand bound at the potential binding site. Also, I have found that there are two more ligand molecules bound along the path from solvent to the binding site. I think this can enrich the ligand to binding site, enhancing the local concentration of the ligand, thus reducing the Km of the ligand. I am wondering if anybody can give some suggestions on how to solve this problem clearly. If there is any similar case, it will be better. Thank you in advance. Best wishes, Yingjie Yingjie PENG, Ph.D. student Structural Biology Group Shanghai Institute of Biochemistry and Cell Biology (SIBCB) Shanghai Institute of Biological Sciences (SIBS) Chinese Academy of Sciences (CAS) 320 Yue Yang Road, Shanghai 200031 P. R. China 86-21-54921117 Email: [email protected]
