I wouldn't use any stock solution that has been sitting around for a year at 4C. Why take the risk that something has happened with it/grown in it? Use a freshly made solution and do not store it at 4C.
Bert van den Berg University of Massachusetts Medical School Program in Molecular Medicine Biotech II, 373 Plantation Street, Suite 115 Worcester MA 01605 Phone: 508 856 1201 (office); 508 856 1211 (lab) e-mail: [email protected] http://www.umassmed.edu/pmm/faculty/vandenberg.cfm -----Original Message----- From: CCP4 bulletin board on behalf of deliang Sent: Wed 2/25/2009 9:06 PM To: [email protected] Subject: [ccp4bb] off-topic detergent hydrolysis? Hi there, I am using a stock solution of Octyl-Glucoside to crystallize my membrane protein, which was made ~1 year before and kept at 4c . I wonder whether OG can be hydrolysed under this condition and whether there is a way to test. Many thanks, Deliang
