Does your system eliminate the need for cryoprotection? Also, how do you
time the buffer-removal/freezing steps?
JPK
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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [email protected]
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----- Original Message -----
From: "Watanabe Nobuhisa" <[email protected]>
To: <[email protected]>
Cc: "Jacob Keller" <[email protected]>
Sent: Thursday, March 26, 2009 10:50 PM
Subject: Re: [ccp4bb] Crystal vacuum cleaner
I am not sure this is the answer for your question, but please check,
http://www.nusrc.nagoya-u.ac.jp/WatanabeLab/XtalMount/index.html
We named it as a "loopless" mounting method in the paper, but now we
leave the loop...
The vacuum cleaner I have used for the method is "my mouth". But we have
just developed a semi-automatic equipment. I hope we will be able to
submit the manuscript soon.
Nobuhisa Watanabe, PhD.
===
Synchrotron Radiation Research Center
Department of Biotechnology and Biomaterial Chemistry,
Graduate School of Engineering
Nagoya University
C1-3(651) Furo-cho Chikusa-ku, Nagoya 4648603 Japan
Email: [email protected] Fax: +81-52-789-5286
On 2009/03/27, at 4:43, Jacob Keller wrote:
Dear Crystallographers,
Has anybody ever heard of mounting crystals in tiny crystal-sized
capillaries, such as are pulled by patch-pipet machines, or those used
in microfluidics? The material could be either glass or plastic, and one
could have some method of continuous positive or negative pressure,
perhaps through a hole in the crystal cap. Anyway, once safely inside
the tiny capilary, one could freeze it at leisure, without concern for
evaporation. It would really make harvesting easy--just vacuum up the
crystal, then plop in LiqN2/ propane as per usual. I guess it could also
really be done with appropriate modification of a micro-manipulator.
Jacob
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [email protected]
*******************************************
