Buz Barstow wrote:
Dear All,
I'd like to add substitute a natural base (adenine) for a non-natural
base (2-aminopurine), in a molecular model of an RNA molecule.
I'd like to do this with CCP4 and Coot. Could I ask for some help please?
The "CCP4 way" would be to use sketcher and search for aminopurines.
You will find 2,6-diaminopurine (1AP). Load this up and delete the
6-amino group to convert to 2-aminopurine. Save the coordinates and
dictionary (give it a new name).
Fire up coot and File -> Import CIF dictionary...
Centre on the residue you want to substitute
Extensions -> Modelling -> Replace residue -> 1AP (or whatever you
called it).
(If you have a map loaded when you do this, it will try to fit to that
as a final step)
Paul.
