If you are uncertain, just freeze your buffer :-)
Very old trick from the stone age of crystallography.
Long time ago ~ 1999 I cryoed one crystal with 1.5 M hexanediol but
there was also 10% glycerol around.
Jürgen
On 2 Apr 2009, at 14:56, HanJie_Heng Chiat Tai wrote:
Hi, Jim,
What's the concentration? I know that [hexanediol] between 2.5 - 3.4
M no additional cryoprotectant is required.
But in my case my hexanediol conc is only 2.1 M
Rgds,
HengChiat Tai (HanJie)
> Date: Thu, 2 Apr 2009 13:11:27 -0500
> From: [email protected]
> To: [email protected]
> CC: [email protected]
> Subject: Re: [ccp4bb] cryoprotectant for 1,6 hexanediol
>
> The cryoprotectant is 1,6 hexanediol.
>
> Jim
>
> On Thu, 2 Apr 2009, HanJie_Heng Chiat Tai wrote:
>
> >
> >
> > Hi,
> >
> >
> >
> > I have a crystal grown in 2.1M 1,6 hexanediol/0.1 M tro-sodium
citrate (pH 6.5).
> >
> >
> >
> > What's the cryoprotectant can be used to flash cool this crystal?
> >
> >
> >
> > Any online protein crystal cryoprotectant database or published
literature available I can check with to determine to type and
concentration of the cryoprotectant used for my crystal.
> >
> >
> > HengChiat Tai
> >
> >
> >
> > _________________________________________________________________
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Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Biochemistry and Molecular Biology, W8708
615 North Wolfe Street
Baltimore, MD 21205
Phone: +1-410-614-4742
Fax: +1-410-955-3655
