Hi,
I have a protein-DNA complex grown in 30 -40% MPD/0.2M Ammonium sulfate/5% PEG 400/ 0.1M Tris (8.5). I believe that 30%MPD above does not require any cryoprotectant but i hv a problem to loop a crystal up. The crystal always sticks with AmSO4. I tried to add 2ul up to 6ul reservoir solution into the protein drop (1+1 ul). Although AmSO4 was diluted, the crystal was still surrounded by AmSO4. The loop size is 0.1 -0.2mm, the crystal is about 0.1 mm. I tried to grow crystal without AMSO4 or 0.1M AmSO4, but no crystals was shown up. Any suggestion to loop up the crystal without any AMSO4 attached? Regards, HCT (HanJie) _________________________________________________________________ Rediscover Hotmail®: Get quick friend updates right in your inbox. http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Updates1_042009
