[ccp4bb] Making really good chemically competent cells

[Wulf Blankenfeldt]

Dear all,

please excuse the non-CCP4-related question, but maybe there is someone 
out there who can help us.

We are trying to find a recipe for making REALLY good chemically 
competent E. coli for our local cloning facility. What we would like to 
have is more or less:

- 109 cfu/ug DNA
- blue/white selection
- phage resistance
- chemical competence (heat shock transformation)

Our best local methods achieve only about 5*106 cfu, which is too little 
for the cloning method we use.

Any suggestions are highly appreciated.

Thanks in advance,



Wulf