Dear all, i am working on a structure of M.tb, solved at 2.18 A. In my structure there is a density near to the active site and we modelled it as a malonate (C3 O4 H2) molecule acorrding to the density, which was not used in any of our buffer except we used 10 mM DTT, 1.6 M Ammonium sulphate, 100 mM citric acid.
Is their any possible way to check for unknown densities or can any one suggest me for any possible compound which can be similar to the malonate or can be formed from above mentioned solutions (byproduct). Thanks Rajan -- ************************************************ Current Address: Rajan Vyas Research Scholar Deptt. of Biotechnology Panjab University Chandigarh, India 160014 Mob. +919417374197 Fax: +91-172-2625254 ************************************************
