If you go to this web-page: http://www.embl-hamburg.de/ExternalInfo/Research/Sax/user_info.html there is ashort summary of the requirements for a SAXS experiment. Shortly the sample needs to be monodisperse (>90%) and you will need a range of concentrations roughly from 0.5/1.0mg/ml to 10mg/ml
Marjolein Thunnissen Phone +46-(0)46-22 24584 Associate Professor Fax +46-(0)46-22 24692 Dept of Molecular Biophysics, Lund University http://www.mbfys.lu.se PO-Box 124 S-221 00 Lund, Sweden Scientific coordinator I911 (Max-lab): MAD and fixed-wavelength stations for macromolecular crystallography ________________________________________________________________________ ________________________________________ From: CCP4 bulletin board [[email protected]] On Behalf Of Li Sheng [[email protected]] Sent: Thursday, April 23, 2009 1:19 PM To: [email protected] Subject: [ccp4bb] protein purity in SAXS Dear all, Is there anyone with experience in small angle x-ray scattering? Could anyone please tell me the requirements for protein sample purity and concentration? Thanx in advance. Sincerely, Li __________________________________________ Email:[email protected] Institute of Biochemistry and Cell Biology Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue-Yang Road, Shanghai 200031, China Tel: +86-21-5492-1217 __________________________________________
