Sanjiv Kumar wrote:
I have not tried purifying this protein either with detergent or with
/Guanidine Hydrochloride. I can try with both /Guanidine HCl and
detergent. Since I got good purification with the 8M urea, I was
thinking if this protein could be refolded and used further. Isn't
removal of detergent more difficult then Urea?
Sanjiv Kumar
Lab. No. 411,
Functional Genomics Unit,
Institute of Genomics and Integrative Biology,
New Delhi-110007
India
Is it a transmembrane protein or not? or just a protein associated to
the membrane (with a GPI anchor for example)?
If it is a transmembrane protein, you should try to use detergents for
extraction and during the purification.
David
--
David Cobessi
Institut de Biologie Structurale
41, Rue Jules Horowitz
38027 Grenoble Cedex-1, France
Tel:33(0)438789613
33(0)608164340
Fax:33(0)438785122
