Sorry Charlie for the late reply.
I'm using the CFX96 5 channel with Sypro and other dyes. I tested the
machine against the Eppendorf product nd decided to go for the Biorad
version as running identical samples gave better results on the
Biorad. This could be a machine specific problem but on a 96 well
plate the detection level was ~1.5x better, you can use the HEX
channel for detection.
Jürgen
On 29 Apr 2009, at 03:06, Charlie Bond wrote:
Hi Folks,
Has anyone here had any success running a thermofluor (differential
scanning fluorimetry) experiment on a BioRad CFX96 with SYPRO orange
as
the fluorophore?
Our local 'technical expert' tells us it should work and gave us some
settings to test, but was very sketchy about any detail, and in a test
we ran we got some strange results (every well that contains SYPRO
orange - even those with no protein - have a peak in fluorescence at
58oC).
Before we tinker, or go and persuade a friendly molbiol lab to let us
play with the filters in their iq5, I thought I'd see if anyone here
had
any advice.
I'll summarise any responses I get.
Cheers,
Charlie
--
Charlie Bond
Professorial Fellow
University of Western Australia
School of Biomedical, Biomolecular and Chemical Sciences
M310
35 Stirling Highway
Crawley WA 6009
Australia
[email protected]
+61 8 6488 4406
-
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Biochemistry and Molecular Biology, W8708
615 North Wolfe Street
Baltimore, MD 21205
Phone: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-3655
http://web.me.com/bosch_lab/
