Dear All:
Recent we expressed one protein using mammalian cells but this protein
was highly glycosylated (30% of the total molecular weight).
We want to remove some carbohydrate by enzymatic digestion for the
convenience of crystallization. However, this protein tends to aggregate at 37
degree.
I am not sure whether EndoH and PNGase can work at 4 degree.
Can anyone give some suggestion?
Thanks a lot,
Jerry McCully
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