I'm not quite sure what you want, but I have a series of vectors
encoding various N-terminal tags and fusions, all followed by a TEV
site. They have an MCS standard to many pET vectors. Therefore, they
are designed to clone your gene in using the NdeI site at the 5' end
(which will, after proteolysis, leave you with GH at the N-terminus of
your protein). Other restriction enzymes in the MCS can be used, but
more amino acids will be left at your N-terminus.
I've used WatCut (from the U. Waterloo) for the silent mutagenesis
question: http://watcut.uwaterloo.ca/watcut/watcut/template.php
Best,
Cynthia
On Jun 5, 2009, at 5:41 PM, Jacob Keller wrote:
Dear Crystallographers,
Does anybody have a TEV-protease-site-coding nucleotide sequence
with a commonly-used restriction site in it, preferably right at the
end? Alternatively, does some somebody know of a program to
determine all equivalent codon permutations for a small coding
region, filtered for resulting restriction site possibilities? It
seems like it would be an easy enough script to write...
(I have already done some googling around for such a program, with
not much luck.)
Jacob
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-kell...@northwestern.edu
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