Hi, Amit If you mostly got clear drops, you might first increase your protein's concentration before you try anything else. Of course, I assume you have a lot of protein, since you are think about doing lysine methylation.
Best, Nian On Wed, Jun 10, 2009 at 6:24 AM, amit sharma<[email protected]> wrote: > Dear All, > > I have a trimeric molecule (~39 kDa, pI=9.1) carrying 15 lysines in each > monomer. I have tried several crystallization screens, however, I mostly get > clear drops. The only conditions that I tend to get precipitates in, are > the ones carrying citrate. I am beginning to methylate the lysines or > remove a few of those by mutagenesis. > > I would be grateful if somebody could share their experience in > crystallization of proteins with a high number of lysines. Also, any inputs > on strategies to crystallize such molecules would be greatly appreciated. > > Many thanks in advance. > > -- > Amit Sharma
