Dear all,
I have a data set which is highly anisotropic (3.5-4A in one direction
and 2A in the other) and huge amount of scattering in some rotation
ranges. I know there is the anisotropic correction server which I can
use to do scaling to the weaker data and get it into a kind-of isotropic
set. I would like to seek advise on how best to integrate the raw data.
Obviously I don't want to throw away the high-resolution data. If I
process it in mosflm to 2.0A, there is got to be a lot of profile
averaging in the areas that there are very few or no spots. What is the
best practice in integrating these data? Shall I just ignore the mosflm
warnings and take it as a matter of fact that I have to live with for
these poor profiles? In the mosflm user guide, it suggests processing
isotropically (because using "anisotropic resolutions" to integrate data
in mosflm will lead to huge incompleteness and refinement programs will
later complain) but do postrefinement with anisotropic resolutions. How
do I do that?
Please share your experiences. Cheers.
Wai
--
Yu Wai Chen, PhD........................................Lecturer
King's College London, Randall Division +44-207-848-8206
New Hunt's House, Guy's Campus, London SE1 1UL, U.K.
