Hi All,
I am working with DNA-Protein complex and getting 3D crystals (~15% PEG 3350,
100mM Ca-acetate, pH 7.4) in Orthorhombic system.
But in one case (different DNA length) it is always coming as a needle cluster
or thin flake (20-25% PEG 3350). I took one needle and solved the structure
(res 3.2A) in Triclinic system (half cell size that of Orthorhombic) two
monomers in assym unit with little bit moderate R-values.
Now my question is - would it be possible to play with the crystallization
condition to get a 3D crystal? Though I have tried some.
Any idea or suggestions will be appreciated.
Regards
Surajit
