Dear members,
I have protein crystals grown in a condition with Isopropanol (13%) and
citric acid (0.2 M). When I transfer the crystals to cryo-buffer (30%
glycerol or 25% ethyleneglycol with 0.25 M citric acid and 13%-15%
Isopropanol), small bubbles appear on the surface of the crystals. These
tiny bubbles sometimes disappear in few seconds and sometimes not. Crystals
are exposed to air only for 2-3 seconds in the process of transfer. I am
worried if the formation of these bubbles is due to evaporation of
isopropanol form the crystal and if it affects the crystal lattice. These
crystals diffract X-rays upto 4 angstroms. Any ideas how to avoid this
problem?
Thank you
Kumar
