Also MALDI-TOF can be used to help identify the lipid (with standards). I vaguely remember one would extract the lipid, from the protein, using organic solvents then apply that to the MS. When I did it (about 10 years ago) I used non plastic vials and a Hamilton syringe so as not to contaminate the sample with plastics.
Hope that's useful! Gina -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Soisson, Stephen Sent: Tuesday, July 07, 2009 2:01 PM To: Subject: Re: [ccp4bb] Lipid content/removal Activated charcoal (see serum albumin structure references) can remove fatty acids. Good luck- Steve -----Original Message----- From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of Jacob Keller Sent: Tuesday, July 07, 2009 1:01 PM To: [email protected] Subject: [ccp4bb] Lipid content/removal Dear Crystallographers, does anybody know of a simple, fast way to determine whether a protein sample contains lipid, and roughly to determine the quantity? As corollary, does anybody know of a quick way to extract/remove the putative lipid? In my case, I am working with an extremely robust/stable soluble protein which may contain bacterial lipids. Thanks for your consideration, Jacob Keller ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: [email protected] ******************************************* Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system.
