Dear all,
sorry for the off-topic question.
We would to start using fluorescence polarization anisotropy to study
protein-protein interaction.
We'd like to gather information about the experimental setup, in
particular:
1. best fluorescent dye according to the molecular weights of the
interacting partners (exc/em wavelength difference, fluorescence
lifetime);
2. preferrable conjugating methods (cys, amino derivatization, else?);
3. any other comment and suggestion.
Thanks in advance,
best regards,
Sebastiano and Marina
--
Sebastiano Pasqualato, PhD
IFOM-IEO Campus
Dipartimento di Oncologia Sperimentale
Istituto Europeo di Oncologia
via Adamello, 16
20139 - Milano
Italy
tel +39 02 9437 5094
