Dear ALL:
Thanks a lot for the input about EPMR statistics. After struggling for
several days, I finally got a promising solution from Phaser with a partial
poly-Ala model with 50% completeness.
two molecules in one ASU:
solu set RFZ=4.2 TFZ=5.9 PAK=0 LLG=23 RFZ 3.8 TFZ=19.0 PAK=3 LLG=110 LLG=123
After some initial refinement, some side chains have been assigned with
R-factor 50% and R-free 52%. In addition, there are some extra density at two
termini.
We tried NCS averaging to improve the map but it did not help that much.
Now we are trying to build more residues in with 2.6A data.
Any suggestions will be highly appreciated.
Jerry McCully
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