Joe schrieb:
Hi,I try to crystallize a protein/DNA complex. Is there a general guideline about size / affinity / symmetry of the DNA site? My understanding is that the shorter DNA size preserving strong binding affinity is the better. Is it right? The protein is a homodimer. I have options of using the native asymmetric DNA binding site or a symmetric site derived from sequence concensus. I will try both but I would like to hear your expert comments also. Thank you in advance.Best, Joe
Hi Joe, check out the references given in CCP4 wiki @ http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Crystal_growth:_Protein-DNA_complexes HTH, Kay -- Kay Diederichs http://strucbio.biologie.uni-konstanz.de email: [email protected] Tel +49 7531 88 4049 Fax 3183 Fachbereich Biologie, Universität Konstanz, Box M647, D-78457 Konstanz This e-mail is digitally signed. If your e-mail client does not have the necessary capabilities, just ignore the attached signature "smime.p7s".
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