Dear All,
I have a PhD studentship to work with me in Grenoble in
collaboration with Jon Waltho's group in Sheffield, where the student
will be registered. I would be grateful if you could pass on the advert
below to any potential candidates. Many thanks, yours, Matthew.
*PhD Thesis Student (m/f)*
*Subject: **Dissection of the mechanism of phosphoryl transfer --
structural studies by X-ray crystallography and ^19 F-NMR***
*General Framework:*
A PhD studentship is available immediately in the Structural Biology
Group at the ESRF (http://www.esrf.fr/UsersAndScience/Experiments/MX) .
The goal is to further the knowledge of the mechanism of phosphoryl
transfer as catalysed by phosphoryl transferase enzymes. To reveal the
fine details of the reaction mechanism you will study enzyme -
transition state analogue complexes by combining high-resolution X-ray
crystallography with solution ^19 F --NMR. The combination of these
techniques has already provided a model for the reaction mechanism of
b-phosphoglucomutase and the study will now be extended to systems, in
particular protein kinases, that are important as potential targets in
the treatment of cancer. The studentship is based in the Structural
Biology Group at the ESRF which provides state of the art facilities
through the Partnership for Structural Biology (www.psb-grenoble.eu
<http://www.psb-grenoble.eu/>). The work is a collaboration with the
group of Prof. Jonathan Waltho, Department of Molecular Biology and
Biochemistry, University of Sheffield, UK (www.shef.ac.uk/mbb/
<http://www.shef.ac.uk/mbb/>) where the ^19 F --NMR experiments will be
conducted. **
*Description of the thesis work:*
The studentship will provide extensive training in Molecular Biology and
two important techniques in Structural Biology -- X-ray Crystallography
and NMR spectroscopy. Specific training will be given in:
* Cloning genes and site directed mutagenesis
* Protein expression and purification
* Crystallisation and crystal structure determination of proteins in
complex with metallofluorides
* ^19 F --NMR spectroscopy of protein metallofluoride complexes
*Place of Work:* ESRF in Grenoble
*Supervisors:* Dr. Matthew Bowler, T: +33 (0)4 76 88 29 28,
email: [email protected] <mailto:[email protected]>
Prof. Jonathan Waltho, T: +44 (0)
114 222 2717, email: [email protected]
<mailto:[email protected]>
*General Conditions:*
You should hold a degree such as a BSc, MSc, Master 2 de Recherche,
Laurea or equivalent in Biochemistry, Chemistry or Physics allowing
enrolment for a PhD. Contract of two years renewable (subject to
satisfactory progress) for one year. Gross salary around 2181 EUR/month.
(The applicant will be responsible for arranging his/her academic
registration and for paying the fees (if any)). The ESRF is an equal
opportunity employer and encourages applications from disabled persons.
*If you are interested, please send us a fax (+33 (0)4 76 88 24 60) or
an e-mail ([email protected] <mailto:[email protected]>) with your
address, and we will provide you with an application form. Or print out
an application form on the World Wide Web
http://www.esrf.fr/Jobs/Applying. In addition to the application form,
you should provide us with a detailed CV and the names of two referees.//*
*Ref. **CFR349** - **Deadline for returning application forms: **27
September 2009*
--
Matthew Bowler
Structural Biology Group
European Synchrotron Radiation Facility
B.P. 220, 6 rue Jules Horowitz
F-38043 GRENOBLE CEDEX
FRANCE
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Tel: +33 (0) 4.76.88.29.28
Fax: +33 (0) 4.76.88.29.04
http://www.esrf.fr/UsersAndScience/Experiments/MX/
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