Hi all, I am now preparing to label my protein with selenium-methionine for phasing. As I have heard that selenium-methionine is prone to oxidation, so is it a must to add reducing agent all along the purification? if so, which reducing agent and what concentration is better ? and any alternatives besides TCEP if i am going to purify it through a Ni column? Thanks a lot.
T.C. Cheng
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