Hi, I am using Refmac 5 for refinement of my protein model (solution by molecular replacement) with TLS restrained refinement option on.Can anyone please guide me about the parameters settings for TLS.I have used defaults .Wether it is good to use multiple TLS restrained refinements rounds.I have observed that R free comes down when I use TLS restrained refinement(after coot building) but decrease is less when I use just restrained refinement. At present my Rfee =0.24 and R=.18
Thanks Manoj
