Hi All
A little off topic, but I am thinking about expressing and purifying 40+
mutants, for an assay, at maybe 1mg total protein each. I'd like the
purification to be quick and easy (ie. one step) but cleaner than just a
6his-tag purification.
Currently, my options are either GST, a longer his tag or a strep-tag.
Any thoughts on the comparison between GST and strep and Ni purifications
would be much appreciated. OR are there any other (better) high affinity
purifications, I've missed, excluding expense Ab resins.
Thanks,
Mark Collins
