Hi all,
I am working with a protein that have 28% similar to my MR template. I have processed data in HKL2000 for one of my crystal and I got unique sol in space group P212121. with LLG 131 and TFZ score 13.5 I have used buccaneer and coot for model building and my Rfee came to 45%. I used the PDB file from this crystal ( Rfee of 45%) as my MR model for data obtained from another crystal and got sol with TFZ score=40.5 and LLG=2305. I used coot and did a round of refmac refinement now my Rfee is 41%. My concern is a) wether my scheme is good or not because I am afraid that this will increase the model bias. b)Now I am stuck at 41% Rfee I have many chain breaks and loop regions have not good density. I tried TLS refinement it did not help. what else I shall try?? Thanks Manoj PSU Biochemistry
