We have unsuccessfully used in the past (by that I mean less than
stellar incorporation) the Gibco/Invitrogen Sf900-SFM w/o Met/Cys media.
Then we saw Karin Reinisch's paper (Dong et al, Immunity, 2009) and they
used Expression Systems' ESF921. This protocol works for us.
Engin
On 8/18/09 2:49 PM, Brad Bennett wrote:
Hi Riya-
Expression Systems has a protocol for SeMet incorporation (Click on
the "Selenomethionine Incorporation" pdf file at
http://www.expressionsystems.com/?mvcTask=documents) and they
advertise that they do just about anything you would want to do with
insect cells (see
http://www.expressionsystems.com/?mvcTask=contractProduction),
including expression of recombinant protein in insect cells. I promise
that I am not an employee or a shareholder of this company. Just used
their products and I was looking for a SeMet protocol recently, too,
and happen to find theirs online. You can contact me off the board if
you want more details.
HTH-
Brad
On Tue, Aug 18, 2009 at 5:09 PM, riya doreen <[email protected]
<mailto:[email protected]>> wrote:
Hi everyone,
I am looking for a method for expressing selenomethionine labeled
protein in insect cells. Can anyone point me to a suitable
protocol ? Additionally, is anyone aware of companies that may
provide this service for a fee ?
Thanks
--
Engin Özkan
Post-doctoral Scholar
Laboratory of K. Christopher Garcia
Howard Hughes Medical Institute
Dept of Molecular and Cellular Physiology
279 Campus Drive, Beckman Center B173
Stanford School of Medicine
Stanford, CA 94305
ph: (650)-498-7111
