Dear all,
Recently I am secretely expressing an ectodomain of a human membrane protein in insect cells. The expression level is moderately high but the target band in the SDS-PAGE gel seems smearing and this is cosistent with the western blot results. Does it result from the heterogeneous glycosylation of the protein? My protein contains quite a lot of N and O linked polysaccharide chains and they are important for the function of the protein. Does the heterogeneously glycosylated protein affect the crystallization? Does anyone have the experience of crystallizing such kind of glycoprotein? Thanks in advance! Ru Heng _________________________________________________________________ 上Windows Live 中国首页，下载最新版Messenger！ http://www.windowslive.cn
