James,
I think the standard suggestions apply. Try to tweak your
crystallization conditions to get a single crystal (i.e. not a series of
stacked plates). PEG 400 can be a good substitute for MPD, but you
could also try additive screens as well as co-crystallization with
products, cofactors, substrates, analogs, etc. to alter the
crystallization.
Alternatively, seek out a beamline that offers X-ray beam diameters of
5-20 microns. You can survey your entire crystal(s) for very small
diffracting volumes that might only contain a single plate. You may get
lucky.
-Andy
===========================================
Andrew T. Torelli Ph.D.
Postdoctoral Associate
Department of Chemistry & Chemical Biology
Baker Laboratory, Cornell University
Ithaca, NY 14853
===========================================
On 9/3/2009 8:40 AM, james09 pruza wrote:
Deal all,
Sorry for the non-ccp4 query once again.
I need suggestions regarding the improvement in crystal quality. I have
crystallized a protein in MPD. The crystals grow like a thin plates and
the plates are stacked together. So, the mosaicity is very high and also
indexing is difficult even at 2 angstrom data set.
All suggestions are welcome.
Thanks.
James.
