Chris Although generally less popular nowadays, microbatch-under-oil has great advantages for anaerobic work. You can keep your stock solutions in the chamber all the time, so you only need to degas your protein. Microbatch finds roughly as many crystals in screening experiments as vapor diffusion (summary at http://www.douglas.co.uk/mbnvdall.htm ). Of course the oil helps to protect the protein too - generally less is denatured on the surface
For an automatic setup that fits in an anaerobic chamber conveniently see the winning entry in Douglas Instrument's competition (2006), from the University of Georgia, Athens http://www.douglas.co.uk/presentations/winner2_files/v3_document.htm Hope that's useful Patrick -- For information and discussion about protein crystallization and automation, please join our bulletin board at http://groups-beta.google.com/group/oryx_group?hl=en patr...@douglas.co.uk Douglas Instruments Ltd. DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK Directors: Peter Baldock, Patrick Shaw Stewart http://www.douglas.co.uk/ Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 Regd. England 2177994, VAT Reg. GB 480 7371 36 > -----Original Message----- > From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of > Christopher Rife > Sent: 02 September 2009 18:34 > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] anaerobic crystallization > > Hi, > > Does anyone have tips or suggestions for getting started with anaerobic > crystallization? Searching through the archives, I was able to find > some > discussion about various glove box options > (http://tinyurl.com/ccp4-glovebox), but not about the process itself > (we > already have a box). > > To simplify things, would it be worthwhile to perform the initial > screens > in something like the pre-filled Qiagen screens > (http://tinyurl.com/qiagen-xseal)? Do I need to worry about evaporation > while solutions are being degassed (esp volatiles)? Better/easier to > try > microbatch? > > Thanks for any input. > > Chris > _______________________________________________________________________ > _________ > ___________________ > > This is an e-mail from Danisco and may contain confidential > information. If you > are not the intended recipient and you > receive this e-mail by mistake, you are not allowed to use the > information, to > copy it or distribute it further. > Please notify us and return it to Danisco by e-mail and delete all > attachments. > Thank you for your assistance. > _______________________________________________________________________ > _________ > __________________
