The plates I used were from Hampton, 72 well microbatch plate, untreated hydrophobic ones, HR3-087.
I thought about other plates once. But what I had in mind was the thing matters would be the oil since the skin appeared at the interface. So I tried different oils for the plates, including mineral oil, paraffin oil, silicon oil. That didn't help. Maybe it is time to try the treated ones. Thank you, Patrick. Shu On Mon, Sep 7, 2009 at 6:50 AM, Patrick Shaw Stewart <[email protected]>wrote: > Shu XU > > > > Which type of microbatch plates are you using? > > > > I ask because generally with our Vapor Batch plates, skins are much less of > a problem with the “treated”, hydrophilic version. There is still a skin, > but it gets stretched tight > > > > Let me know if you would like some samples of the plates > > > > Best wishes > > > > Patrick > > > > > > -- > > For information and discussion about protein crystallization and > automation, please join > > our bulletin board at http://groups-beta.google.com/group/oryx_group?hl=en > > > > [email protected] Douglas Instruments Ltd. > > DouglasHouse, EastGarston, Hungerford, Berkshire, RG177HD, UK > > Directors: Peter Baldock, Patrick Shaw Stewart > > http://www.douglas.co.uk/ > > Tel: 44 (0) 148-864-9090 US toll-free 1-877-225-2034 > > Regd. England 2177994, VAT Reg. GB 480 7371 36 > > > > *From:* CCP4 bulletin board [mailto:[email protected]] *On Behalf Of *Shu > XU > *Sent:* 06 September 2009 17:21 > *To:* [email protected] > *Subject:* Re: [ccp4bb] How to get rid of the skin in the screening drop > > > > Lower the concentration did help a little bit. I didn't the 6 mg/ml setups, > but still got some skins. > > You are right the skins are more likely to find in the condition of > PEGs...but also find in other conditions. > > Lower the conc. of PEGs is a good advice, and I'll try it. Thanks. > > Shu > > On Sun, Sep 6, 2009 at 10:29 AM, Jeremiah Farelli <[email protected]> wrote: > > This happened to me previously, and the only thing that worked for me was > to > lower the protein concentration. I was setting my protein up at 10 mg/mL, > only to find skins on nearly all the drops. I would damage the crystals > trying to get them out of the drops... I lowered my protein concentration > to > 7.5 mg/mL... the crystals still grew, and the skin didn't. > > This seems to happen a lot when you set up crystals with PEGs, so you might > be able to lower the concentration of PEG as well. > > >
