Hi! On Pet, rujan 11, 2009 15:37, sajid akthar wrote: > I have some density and I can fit a peg molecule in their. I am confused > and when I search database for peg3350, I have no hit. How can I build a > model for Peg 3350. I found some PDB's with peg; but they they have used > peg400; Is there any difference in the model between peg3350 and peg400.
PEG 3350 (as other PEGs) is a mixture of many polymers with different numbers of -CH2CH2O- mers, but with the average relative molecular mass of 3500. In electron density maps, one can usually see only a patch of PEG polymeric chain bound to protein molecules, so one needs to find out (i.e. to estimate) what is the proper length of the bound PEG fragment. > Can any one define where the peg ion could bind. Have a look at http://dx.doi.org/10.1524/zksu.2006.suppl_23.613. It might give you an idea where PEG molecules usually bind on protein molecules. Regards, Dalibor -- Dalibor Milic Laboratory of General and Inorganic Chemistry Department of Chemistry Faculty of Science University of Zagreb Horvatovac 102a HR-10000 Zagreb Croatia phone: +385 1 460 6377 fax: +385 1 460 6341 e-mail: dmi...@chem.pmf.hr
