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Atul, Reasons could be so many. Since you say that the His-tag is still present in your protein try to remove it and see if it can make a difference. OR Also, try seeding. You don't say what your protein concentration is. If it is too high, reduce it or vice-versa. Also, in general if crystals appear in one organic acid condition, you can try in others like citric acid and very importantly Sodium MALONATE. Play little bit with pH. Good luck Anthony ----------------------------------------------------- Dr. Anthony Addlagatta Scientist E1 Indian Institute of Chemical Technology [IICT] Tarnaka, Hyderabad AP-500 607, INDIA Tel:91-40-27191583 ---------- Original Message ----------- From: atul kumar <[email protected]> To: [email protected] Sent: Wed, 30 Sep 2009 12:27:44 +0530 Subject: [ccp4bb] improvement of tiny protein crystals > *********************** > This message has been scanned by the InterScan for CSC SSM at IICT and found > to be free of known security risks. *********************** > > hi all > sorry for asking non ccp4bb question. > i am trying to crystallise phosphatase protein,it has histag.I am getting > very tiny crystals into .1m hepes ph7.4,1m Na-K tartrate.I tried > additives(MPD, > ethanol,propanol)for improvement of crystals,but didnt get any success.I have > tried both sitting and hanging drop conditions.Does anyone have suggestion > for > the improvement of these crystals? thanks Atul Kumar > > -----Original Message----- > From: CCP4 bulletin board on behalf of Jessica Gilmore > Sent: Wed 9/30/2009 2:44 AM > To: [email protected] > Subject: [ccp4bb] Bioinformatics Technician Position - Center for the Study > of > Systems Biology, Georgia Tech > > Bioinformatics Technician Position Available - Center for the Study of > Systems Biology, Georgia Tech > > Job Summary > Assists research staff in the integration, management and analysis of > biological data, with a focus on the development, updating and > maintenance of internal and public databases dealing with genome, > transcriptome, proteome and metabolome annotation results generated in > silico. > > Job Qualifications > Requires a BS, MS, or PhD in Bioinformatics, Computer Science, or > related fields. > Minimum of two years verifiable experience in web site development and > database design. > Experience in scripting languages, preferably in a Unix/Linux > environment. > Familiarity with the use of biological databases. > Demonstrated written and verbal communication skills. > > To apply please email your CV to: [email protected] ------- End of Original Message ------- This Mail Scanned by ClamAV and Spammassassin
