I agree. It cannot be said too often that the object of refinement is
not to minimise the R-factor!
(& while I'm at it, freeR has nothing to do with model bias (which is
largely imaginary anyway, only a problem with low resolution molecular
replacement))
Phil
On 19 Oct 2009, at 17:07, Ed Pozharski wrote:
My resolution is 1.6A although I have cut it to 1.8A to bring the
R-factor down. I've been performing restrained refinement in refmac5
using the default settings. The solvent content is 40%
This sounds fundamentally wrong. Even the "Rmerge reduction by
cutting
resolution" practice is questionable due to strong dependence of the
Rmerge on redundancy, but to cut resolution to get better Rfactor in
refinement... Nobody throws away the lower concentration (and thus
noisier) data in Bradford assay, although I am sure that the
appropriate
"R-factor" would definitely go down.
