On Monday 19 October 2009 12:43:43 Jerry McCully wrote: > > Dear All: > > I got a problem with a data set in R32 space group with a resolution > of 2.6. > Pointless indicated that the space group was R32 and phenix triage showed > that there was a pseudotranslation in the ASU. > > My protein is a hexamer and the matthew coeff. showed that there should just > be two monomers in one ASU of R32. > > However, phaser in R32 and Molrep in R32 gave different solutions because > Molrep used the pseudo-translation. I am not sure which one was correct > because refinement of both solutions get stuck around 35% for Rfactor. > > I tried the molecular replacement in P1 and I got good solutions in Phaser. I > think they are correct solutions because my protein was a hexamer and the > crystal packing in P1 was formed by hexamers. > > How can I get correct solutions in R32 and finish the refinement?
Did you try using the hexamer as your molecular replacement probe? -- Ethan A Merritt Biomolecular Structure Center University of Washington, Seattle 98195-7742
