IB's precipitate in a few minutes at max. speed of tabletop Eppendorf. Almost any speed above 5-6K in a larger rotor will precipitate them in 10-20 minutes, provided that the density of your IB wash is normal (i.e. you're not using 70% sucrose, Renografin, or Cesium Chloride etc).
In other terms, no worries. You have the technology. Artem _____ From: CCP4 bulletin board [mailto:[email protected]] On Behalf Of megha goyal Sent: Tuesday, October 27, 2009 10:21 PM To: [email protected] Subject: [ccp4bb] Inclusion bodies centrifugation Dear All, Our protein is expressed as inclusion bodies and I want to separate inclusion bodies from E.coli from the cellular debris after lysis of the cells by sonication. Can I do this by normal centrifugation? and if yes, at what speed? Our centrifuge has maximum speed of 14000 rpm. Can we do the separation using this centrifuge and if so how. Thanking in anticipation.
