Hi,
We would like to design a self-cleaving tag. It will be similar to the one
Roger Tsien has developed but I hope to find one that works in the opposite
way.
Lin, M.Z. et al. A drug-controllable tag for visualizing newly synthesized
proteins in cells and whole animals. Proc. Natl. Acad. Sci. USA 105, 7744–7749
(2008).
Tsien used the NS3 protease which is inhibited by a cell-permeant drug. The tag
will be cleaved in the absence of this drug. I would like to find a small and
monomeric protease which is inactive until a drug or a co-factor is applied.
Any suggestions will be highly appreciated.
Best,
Daniel
