Radovan Spurny wrote:
I would like to ask for recommendations and tips how to use lipids for
crystallization of membrane proteins. Especially I am interested about
the solubilization of lipids (E.coli lipids, POPC, POPE, POPG) and how
to mix them with protein.
Thanks a lot.
Have a look at:
http://www.ncbi.nlm.nih.gov/pubmed/12702760
A defined protein-detergent-lipid complex for crystallization of integral membrane
proteins: The cytochrome b6f complex of oxygenic photosynthesis.
Zhang H, Kurisu G, Smith JL, Cramer WA.
Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5160-3. Epub 2003 Apr 17.
________________________________________
Radovan Spurny, PhD
Laboratory of Structural Neurobiology
Division of Pharmacology
Campus Gasthuisberg, ON1
Herestraat 49, PB 601
B-3000 Leuven
Belgium
e-mail: [email protected]
<mailto:[email protected]>
web: www.xtal.be <http://www.xtal.be/>
