Try reductive methylation of the lysines: Walter, T. S., Meier, C., Assenberg, R., Au, K. F., Ren, J., Verma, A., Nettleship, J. E., Owens, R. J., Stuart, D. I. & Grimes, J. M. (2006). Structure, 14, 1617–1622.
Cheers, Stephen 2009/11/2 Jan Rash <[email protected]>: > Dear All, > > I have a question regarding the crystallization of lysine and arginine rich > protein around 13%. So far our attempts to crystallize this protein have not > been successful although the secondary structure predictions, CD > spectroscopy measurements clearly show that this protein is folded. I > presume that these lysine and arginine are the sources of the local > flexibility in the protein even though the protein is globular overall. > Moreover, my attempts to crystallize the limited proteolysis fragments also > did not achieve crystals. I have also tried the crystallization with its > binding partners and could not succeed. I think any compound that binds to > the lysine/arginine side chains might affect the crystallization process > thereby reducing the internal flexibility of protein. Can anybody suggest > some effective strategy for the crystallization? > > > > Thanks > > Umar > > -- Dr Stephen Graham 1851 Research Fellow Cambridge Institute for Medical Research Wellcome Trust/MRC Building Addenbrooke's Hospital, Hills Road Cambridge, CB2 0XY, UK Phone: +44 1223 762 638
