Hi Mattias,
For maximizing the anomalous scattering of chlorides, calcium and
sulfate I always collect highly redundant data (say 7 fold) at 7keV
(1.77A). As for the optimal wavelength for S-SAD I would point you to
the following publication:
http://journals.iucr.org/d/issues/2005/09/00/dz5049/dz5049bdy.html
Cheers, Matt.
Matthias Zebisch wrote:
Dear bb!
What is the optimal wavelength for Sulfur SAD phasing?
Is it 1.9A or should one go below that to reduce absorption/damage.
Also, would the same wavelength be appropriate to maximize anomalous
scattering to position chlorides, calcium, sulfate in already phased
structures?
Thanks in advance,
Matthias
--
Matthew Bowler
Structural Biology Group
European Synchrotron Radiation Facility
B.P. 220, 6 rue Jules Horowitz
F-38043 GRENOBLE CEDEX
FRANCE
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