Maybe... Depending on the energetics - it could stall the translation -
or prevent initiation if it is at the start of the ORF. If it is in the
middle - you might be ok... Usually the gene synthesis company can
recommend alternate sequences that do not run into this problem or rare
codon issues...
You may wish to see:
Protein Expr Purif. <javascript:AL_get(this, 'jour', 'Protein Expr
Purif.');> 2006 Feb;45(2):374-80. Epub 2005 Aug 8.
Rapid and easy thermodynamic optimization of the 5'-end of mRNA
dramatically increases the level of wild type protein expression in
Escherichia coli.
Cèbe R
<http://www.ncbi.nlm.nih.gov/pubmed?term=%22C%C3%A8be%20R%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstract>,
Geiser M
<http://www.ncbi.nlm.nih.gov/pubmed?term=%22Geiser%20M%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVAbstract>.
You mileage may vary....
Ezra
Sangeetha Vedula wrote:
Dear bb-ers,
I am trying to have a gene synthesized and found out that it forms an
11-bp hairpin. Does that complicate expression? Would it be better to
try and disrupt it by altering codon usage to improve expression?
Thank you in advance,
Sangeetha.
