Hi all, We use 8M urea solubilization buffer for our protein in inclusion bodies and recommended temperature is 10-15ยบ C. but in 8M conc the urea does not dissolve and is in crystalline form only, will it have any effect on solubilzation efficiency. Our solubilization time is 1 Hr and after that we centrifuge and use the supernatant for refolding via dialysis. however the pellet after centrifugation of solubilzation show presence of our protein on sds page analysis. what should we do so that the process of solubilization is complete and our protein is not lost in pellet.
thanks in anticipation. meg
