Dear Subbu,
One more thing you can do is to search with an ensemble of structures (4
in your case) for molecular replacement.
Fred.
Narayanan Ramasubbu wrote:
Deal All:
I have a 2.0 A data for a SeMet protein (native crystal not available
yet!) that has 6 Se sites.
The cell comes out to be 65 67 101 and the angles are all very close
to 90. The data set was collected in house with Cu 1.5418 A
We integrated and scale in orthorhombic and the statistics are
reasonable.
There are 4 homologous structures and all of them have a sequence
identity of 15-16 %.
I have tried Phaser, AMoRe, EPMR with varying templates
with polyala, polyser and varying the resolutions.
I have also done a modeller and found best match with one of the four
structures. I have used this derived model as well for input.
I would like to know whether there other options (I am working on
getting a good synchrotron data set at the peak wavelength for Se).
Thanks
Subbu
PS: I am also trying P1 just in case.
