Dear CCP4BBers, Sorry for the non-CCP4 question. I intend to set up some trials for protein-DNA complexes. In this regard, I have a few queries:
1. After annealing the oligos, how do I ensure that what goes into trials are only the annealed dsDNA and not other forms such as ones carrying hairpin loops. 2. Should I visualize it on the agarose gel and then try to get the dsDNA band? Sorry for these queries as I have little idea about this. Many thanks in advance. Cheers -- Amit Sharma, Ph.D. Postdoctoral Fellow, Department of Biophysics, Johns Hopkins University, Baltimore, MD21218
